Recipe
How this sample was made, step by step · total growth 3 days-
1
Substrate
- 100 wt%
- Hemp shiv (untreated) (average approx. 10 × 2 mm; ProRep (UK))
- Pre-treatment
- soaked in water for 24 h, drained
-
2
Sterilisation
- Method
- Autoclaving
- Details
- 121 °C, 15 psi, 30 min (PP autoclave bags)
-
3
Inoculation
- Fungus
- Lentinus tigrinus
- Inoculum
- Grain spawn
- Rate
- 10 wt%
-
4
Growth in mould
- Mould
- cylindrical moulds H 5 cm × Ø 8 cm, cleaned with 70 % isopropanol, covered with Parafilm
-
5
Post-growth
3 days- Where
- demoulded
- Temperature
- 23 °C
-
6
Inactivation
- Method
- Oven drying
- Temperature
- 50 °C
- Duration
- 48 h
-
7
Specimen & testing
- Geometry
- cylinder Ø 8 × 5 cm
- Measurements
- 1
Post-growth 3 days
Steps marked “not reported” were not stated in the source. Suggest missing details
Highest thermal conductivity of the 18 strains tested.
Measurements
Thermal
| Property | Value | n | Test standard | Conditions | |
|---|---|---|---|---|---|
| Thermal conductivity λ | 0.0451 ± 0.001 W/(m·K) | 4 | heat flow meter (Thermtest HFM-25), steady state | 1 week at approx. 20 °C / 50 % RH; mean temperature 20 °C, ΔT 20 K 4 specimens × 5 measurements |
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Production
Substrate mix
Fungus & inoculation
- Fungus
- Lentinus tigrinus
- Inoculum type
- Grain spawn
- Inoculation rate [wt%]
- 10
- Sterilisation
- Autoclaving
- Sterilisation – details
- 121 °C, 15 psi, 30 min (PP autoclave bags)
Growth
- Post-growth after demoulding [days]
- 3
- Temperature [°C]
- 23
- Mould / mould material
- cylindrical moulds H 5 cm × Ø 8 cm, cleaned with 70 % isopropanol, covered with Parafilm
Post-processing
- Inactivation
- Oven drying
- Inactivation – temperature [°C]
- 50
- Inactivation – duration [h]
- 48
Specimen
- Specimen geometry
- cylinder Ø 8 × 5 cm
Notes
Spawn on brown rice (food grade); substrate:spawn = 10:1. In the mould for 3–7 days depending on species until fully colonised, then 3 days in larger pots. Four biological replicates with different spawn batches. Density determined per ASTM C303 (single values only in Fig. 2b).
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