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Flax, chopped · T. versicolor (FC)

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Trametes versicolor (M9912-5LSR-2 O447A) · Vrije Universiteit Brussel (BE)

Recipe

How this sample was made, step by step · total growth 16 days
  1. 1

    Substrate

    100 wt%
    Flax hurds, chopped (≤ 5 mm; Jopack bvba, Rumbeke (BE))
    Pre-treatment
    soaked in water for 24 h, rinsed, blended with water for 10 min (Emerio BL-108862), drained over a 5 mm sieve, dried at 30 °C for 24 h
  2. 2

    Sterilisation

    Method
    Autoclaving
    Details
    121 °C, 20 min (fibres only)
  3. 3

    Inoculation

    Fungus
    Trametes versicolor · M9912-5LSR-2 O447A
    Inoculum
    Grain spawn
    Rate
    50 wt%
    Water content
    70 %
  4. 4

    Growth in mould

    8 days
    Mould
    two-part PVC tube mould covered with perforated cellophane; grown in a filter micro box
    Temperature
    28 °C
  5. 5

    Post-growth

    8 days
    Where
    demoulded
  6. 6

    Inactivation

    Method
    Oven drying
    Temperature
    70 °C
  7. 7

    Specimen & testing

    Geometry
    cylinder Ø 75 × 37.5 mm (compression), Ø 63 × 200 mm (λ), Ø 20 × 100 mm (water)
    Dry density
    65.8 kg/m³
    Mass loss
    81.2 %
    Shrinkage
    13 %
    Measurements
    5

Steps marked “not reported” were not stated in the source. Suggest missing details

Flax hurds < 5 mm, chopped.

Measurements

Mechanical

PropertyValuenTest standardConditions
Compressive modulus 1.18 MPa 3 ASTM D3501 (tangent modulus) 25 °C / approx. 50 % RH
5 mm/min, test up to 70–80 % strain; mean value from text, scatter only in Fig. 4
edit

Physical properties

PropertyValuenTest standardConditions
Density 65.8 ± 4.2 kg/m³ 3 ISO 9427:2003
compression specimen
edit

Moisture & water

PropertyValuenTest standardConditions
Capillary water absorption rate (sorptivity) 0.0113 ± 0.002 mm/√s 3 ASTM C1585 (partial immersion), 20 °C
specimen Ø 20 × 100 mm, density 137.5 ± 2.9 kg/m³
edit
Water absorption after 24 h (partial immersion) 30.28 wt% 3 ASTM C1585 (partial immersion), 20 °C edit

Thermal

PropertyValuenTest standardConditions
Thermal conductivity λ 0.0578 ± 0.002 W/(m·K) 3 ASTM D5334-00 (Hukseflux TP02 needle probe), with deviation in heating current
specimen Ø 63 × 200 mm, density 134.7 kg/m³
edit

Production

Fungus & inoculation

Fungus
Trametes versicolor (M9912-5LSR-2 O447A)
Inoculum type
Grain spawn
Inoculation rate [wt%]
50
Water content at inoculation [%]
70
Sterilisation
Autoclaving
Sterilisation – details
121 °C, 20 min (fibres only)

Growth

Growth in mould [days]
8
Post-growth after demoulding [days]
8
Temperature [°C]
28
Mould / mould material
two-part PVC tube mould covered with perforated cellophane; grown in a filter micro box

Post-processing

Inactivation
Oven drying
Inactivation – temperature [°C]
70

Specimen

Specimen geometry
cylinder Ø 75 × 37.5 mm (compression), Ø 63 × 200 mm (λ), Ø 20 × 100 mm (water)
Dry density [kg/m³]
65.8
Mass loss on drying [%]
81.2
Shrinkage [%]
13

Notes

Recipe: 20 wt% fibres, 70 wt% sterile demineralised water, 10 wt% grain spawn; inoculation rate calculated from this (10/20). Filled into the mould in layers and compacted with a spoon. Demoulded after 8 days and grown for at least 8 more days without mould. Dried for 5–10 h until constant mass. Mass loss = initial moisture content per ISO 16979 (Table 2).

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