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Wheat grain composite, 12 days growth · T. versicolor

✓ Reviewed content

Trametes versicolor (commercial (New Generation Mushroom Supplies)) · RMIT University, Melbourne (AU) et al.

Recipe

How this sample was made, step by step · total growth 12 days
  1. 1

    Substrate

    100 wt%
    Wheat grains (E&A Salce Pty Ltd (AU))
    Pre-treatment
    soaked in Milli-Q water (type 1) for 48 h
  2. 2

    Sterilisation

    Method
    Autoclaving
    Details
    121 °C, 103.4 kPa, 90 min
  3. 3

    Inoculation

    Fungus
    Trametes versicolor · commercial (New Generation Mushroom Supplies)
    Inoculum
    Grain spawn
    Rate
    25 wt%
  4. 4

    Growth in mould

    12 days
    Mould
    sterile plastic moulds 100 × 100 mm
  5. 5

    Inactivation

    Method
    Oven drying
    Temperature
    50 °C
    Duration
    48 h
  6. 6

    Specimen & testing

    Geometry
    100 × 100 mm (cone calorimeter)
    Measurements
    1

Steps marked “not reported” were not stated in the source. Suggest missing details

Wheat grains with T. versicolor, incubated for 12 days.

Measurements

Fire behaviour

PropertyValuenTest standardConditions
Peak heat release rate (pHRR) 180 kW/m² ISO 5660-1 heat flux 50 kW/m², horizontal, 25 mm distance, spark ignition
given in the text as “approx. 180 kW/m²” for 6, 12 and 18 days (wheat grains without mycelium: approx. 200 kW/m²)
edit

Production

Fungus & inoculation

Fungus
Trametes versicolor (commercial (New Generation Mushroom Supplies))
Inoculum type
Grain spawn
Inoculation rate [wt%]
25
Sterilisation
Autoclaving
Sterilisation – details
121 °C, 103.4 kPa, 90 min

Growth

Growth in mould [days]
12
Mould / mould material
sterile plastic moulds 100 × 100 mm

Post-processing

Inactivation
Oven drying
Inactivation – temperature [°C]
50
Inactivation – duration [h]
48

Specimen

Specimen geometry
100 × 100 mm (cone calorimeter)

Notes

Inoculum 25 wt% (reference basis not specified). Incubated under standard atmospheric conditions. Mycelium-rich surface layer 1.2 ± 0.2 mm thick (optical microscopy).

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