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Wheat grain composite, 6 days growth · T. versicolor

✓ Reviewed content

Trametes versicolor (commercial (New Generation Mushroom Supplies)) · RMIT University, Melbourne (AU) et al.

Recipe

How this sample was made, step by step · total growth 6 days
  1. 1

    Substrate

    100 wt%
    Wheat grains (E&A Salce Pty Ltd (AU))
    Pre-treatment
    soaked in Milli-Q water (type 1) for 48 h
  2. 2

    Sterilisation

    Method
    Autoclaving
    Details
    121 °C, 103.4 kPa, 90 min
  3. 3

    Inoculation

    Fungus
    Trametes versicolor · commercial (New Generation Mushroom Supplies)
    Inoculum
    Grain spawn
    Rate
    25 wt%
  4. 4

    Growth in mould

    6 days
    Mould
    sterile plastic moulds 100 × 100 mm
  5. 5

    Inactivation

    Method
    Oven drying
    Temperature
    50 °C
    Duration
    48 h
  6. 6

    Specimen & testing

    Geometry
    100 × 100 mm (cone calorimeter)
    Measurements
    1

Steps marked “not reported” were not stated in the source. Suggest missing details

Wheat grains with T. versicolor, incubated for 6 days.

Measurements

Fire behaviour

PropertyValuenTest standardConditions
Peak heat release rate (pHRR) 180 kW/m² ISO 5660-1 heat flux 50 kW/m², horizontal, 25 mm distance, spark ignition
given in the text as “approx. 180 kW/m²” for 6, 12 and 18 days (wheat grains without mycelium: approx. 200 kW/m²)
edit

Production

Fungus & inoculation

Fungus
Trametes versicolor (commercial (New Generation Mushroom Supplies))
Inoculum type
Grain spawn
Inoculation rate [wt%]
25
Sterilisation
Autoclaving
Sterilisation – details
121 °C, 103.4 kPa, 90 min

Growth

Growth in mould [days]
6
Mould / mould material
sterile plastic moulds 100 × 100 mm

Post-processing

Inactivation
Oven drying
Inactivation – temperature [°C]
50
Inactivation – duration [h]
48

Specimen

Specimen geometry
100 × 100 mm (cone calorimeter)

Notes

Inoculum 25 wt% (reference basis not specified). Incubated under standard atmospheric conditions. Mycelium-rich surface layer 0.9 ± 0.1 mm thick (optical microscopy).

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