Wood + pure mycelium + 7.5 % CNF (90W2.5M7.5)
✓ Reviewed content
White-rot fungus (basidiomycete), undisclosed (Ecovative Design (proprietary)) · University of Maine (US); USDA Forest Products Laboratory (US); Ecovative Design (US)
Recipe
How this sample was made, step by step-
1
Substrate
- 90 wt%
- SPF particleboard particles (spruce/pine/fir) (1.4–3.5 mm; Ecovative Design LLC, Green Island, NY (US))
- 7.5 wt%
- Cellulose nanofibrils (CNF) (3 wt% suspension, fines content > 90 % (< 200 µm); University of Maine, Process Development Center (US))
- 2.5 wt%
- Pure mycelium tissue (aerially grown, dried) (Ecovative Design LLC, Green Island, NY (US))
- Pre-treatment
- grown aerially to a loft height of 75 mm, harvested and dried at 43 °C
-
2
Sterilisation
Not reported
-
3
Inoculation
- Fungus
- White-rot fungus (basidiomycete), undisclosed · Ecovative Design (proprietary)
- Inoculum
- Other
-
4
Growth in mould
- Mould
- aluminium forming box 120 × 120 mm; thickness set by stops
-
5
Inactivation & pressing
- Method
- Hot pressing
- Temperature
- 180 °C
- Duration
- 0.25 h
- Pressure
- 0.4 MPa
-
6
Specimen & testing
- Geometry
- panel 120 × 120 mm, thickness 9.4 mm; specimens 3 × 3 cm (water, internal bond), 12 × 3 cm (bending)
- Dry density
- 600 kg/m³
- Measurements
- 1
Steps marked “not reported” were not stated in the source. Suggest missing details
Group 1: wood particles physically mixed with pure mycelium tissue and 7.5 % CNF.
Measurements
Mechanical
| Property | Value | n | Test standard | Conditions | |
|---|---|---|---|---|---|
| Internal bond strength | 0.06 MPa | 8 | ASTM D1037 (2012), modified | 0.4 mm/min |
edit |
Production
Substrate mix
- 90 %
- SPF particleboard particles (spruce/pine/fir) · 1.4–3.5 mm · Ecovative Design LLC, Green Island, NY (US)
- 7.5 %
- Cellulose nanofibrils (CNF) · 3 wt% suspension, fines content > 90 % (< 200 µm) · University of Maine, Process Development Center (US)
- 2.5 %
- Pure mycelium tissue (aerially grown, dried) · Ecovative Design LLC, Green Island, NY (US)
Fungus & inoculation
- Fungus
- White-rot fungus (basidiomycete), undisclosed (Ecovative Design (proprietary))
- Inoculum type
- Other
Growth
- Mould / mould material
- aluminium forming box 120 × 120 mm; thickness set by stops
Post-processing
- Inactivation
- Hot pressing
- Inactivation – temperature [°C]
- 180
- Inactivation – duration [h]
- 0.25
- Pressing pressure [MPa]
- 0.4
Specimen
- Specimen geometry
- panel 120 × 120 mm, thickness 9.4 mm; specimens 3 × 3 cm (water, internal bond), 12 × 3 cm (bending)
- Dry density [kg/m³]
- 600
Notes
Components mixed for 2 min in a stand mixer (without CNF: the same amount of water added), cold pre-pressed at approx. 400 kPa (solids content 16 % → 38 %), then hot-pressed at 180 °C for 15 min. Density = target value. The mycelium was grown at Ecovative in a proprietary process and dried at 43 °C.
Community feedback
Is something wrong or confirmed by your own tests? Mark the experiment as ✓ correct or ✗ not correct and explain why.
No comments yet.